A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

Anne W. Thompson; Matthew J. Crow; Brian Wadey; Christina Arens; Serdar Turkarslan; Sergey Stolyar; Nicholas Elliott; Timothy W. Petersen; Ger van den Engh; David A. Stahl; Nitin S. Baliga

doi:10.1016/j.mimet.2015.07.009

A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

Anne W. Thompson
, Matthew J. Crow
, Brian Wadey
, Christina Arens
, Serdar Turkarslan
, Sergey Stolyar
, Nicholas Elliott
, Timothy W. Petersen
, Ger van den Engh
, David A. Stahl
, Nitin S. Baliga

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.

Original language	English
Pages (from-to)	74-77
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	117
DOIs	https://doi.org/10.1016/j.mimet.2015.07.009
State	Published - Oct 1 2015

Keywords

Anaerobic microorganism
Controlled-environment sorting
Flow cytometry
Single cells

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10.1016/j.mimet.2015.07.009

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Thompson, A. W., Crow, M. J., Wadey, B., Arens, C., Turkarslan, S., Stolyar, S., Elliott, N., Petersen, T. W., van den Engh, G., Stahl, D. A., & Baliga, N. S. (2015). A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities. Journal of Microbiological Methods, 117, 74-77. https://doi.org/10.1016/j.mimet.2015.07.009

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abstract = "A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.",

keywords = "Anaerobic microorganism, Controlled-environment sorting, Flow cytometry, Single cells",

author = "Thompson, \{Anne W.\} and Crow, \{Matthew J.\} and Brian Wadey and Christina Arens and Serdar Turkarslan and Sergey Stolyar and Nicholas Elliott and Petersen, \{Timothy W.\} and \{van den Engh\}, Ger and Stahl, \{David A.\} and Baliga, \{Nitin S.\}",

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doi = "10.1016/j.mimet.2015.07.009",

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AU - Crow, Matthew J.

AU - Wadey, Brian

AU - Arens, Christina

AU - Turkarslan, Serdar

AU - Stolyar, Sergey

AU - Elliott, Nicholas

AU - Petersen, Timothy W.

AU - van den Engh, Ger

AU - Stahl, David A.

AU - Baliga, Nitin S.

PY - 2015/10/1

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AB - A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.

KW - Anaerobic microorganism

KW - Controlled-environment sorting

KW - Flow cytometry

KW - Single cells

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EP - 77

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

Abstract

Keywords

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