Antisecretory mechanisms of Peptide YY in rat distal colon

Edward E. Whang; Oscar J. Hines; Joseph R. Reeve; Daniel Grandt; James A. Moser; Anton J. Bilchik; Michael J. Zinner; David W. McFadden; Stanley W. Ashley

doi:10.1023/A:1018869116284

Antisecretory mechanisms of Peptide YY in rat distal colon

Edward E. Whang
, Oscar J. Hines
, Joseph R. Reeve
, Daniel Grandt
, James A. Moser
, Anton J. Bilchik
, Michael J. Zinner
, David W. McFadden
, Stanley W. Ashley

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Peptide YY (PYY) is a potent regulator of intestinal secretion. These studies investigated the role of Y1 and Y2 receptor subtypes in mediating the antisecretory effects of PYY on mucosa-submucosa preparations of rat distal colon. Addition of vasoactive intestinal peptide (VIP) to these tissues resulted in a 140 ± 18% increase in basal short-circuit current (I(sc)) and the induction of Cl secretion. VIP-stimulated increases in I(sc) were abolished by the addition of each of PYY, (Pro³⁴)-PYY, a Y1 receptor- selective agonist, and PYY-(3-36), an endogenous Y2 receptor-selective ligand. However, when tissue neural transmission was blocked with tetrodotoxin, neither PYY nor its receptor subtype-selective analogs were able to inhibit VIP-stimulated increases in I(sc). These results suggest that in the rat distal colon, the antisecretory actions of PYY are mediated through a combination of Y1 and Y2 receptor subtypes or through a novel receptor subtype that is unable to discriminate between (Pro³⁴)-PYY and PYY-(3-36).

Original language	English
Pages (from-to)	1121-1127
Number of pages	7
Journal	Digestive Diseases and Sciences
Volume	42
Issue number	6
DOIs	https://doi.org/10.1023/A:1018869116284
State	Published - 1997

Keywords

Colon
Intestinal secretion
Neuropeptide Y
Peptide YY

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10.1023/A:1018869116284

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@article{c47f1a85516745bc91fcb7844e57bce7,

title = "Antisecretory mechanisms of Peptide YY in rat distal colon",

abstract = "Peptide YY (PYY) is a potent regulator of intestinal secretion. These studies investigated the role of Y1 and Y2 receptor subtypes in mediating the antisecretory effects of PYY on mucosa-submucosa preparations of rat distal colon. Addition of vasoactive intestinal peptide (VIP) to these tissues resulted in a 140 ± 18\% increase in basal short-circuit current (I(sc)) and the induction of Cl secretion. VIP-stimulated increases in I(sc) were abolished by the addition of each of PYY, (Pro34)-PYY, a Y1 receptor- selective agonist, and PYY-(3-36), an endogenous Y2 receptor-selective ligand. However, when tissue neural transmission was blocked with tetrodotoxin, neither PYY nor its receptor subtype-selective analogs were able to inhibit VIP-stimulated increases in I(sc). These results suggest that in the rat distal colon, the antisecretory actions of PYY are mediated through a combination of Y1 and Y2 receptor subtypes or through a novel receptor subtype that is unable to discriminate between (Pro34)-PYY and PYY-(3-36).",

keywords = "Colon, Intestinal secretion, Neuropeptide Y, Peptide YY",

author = "Whang, \{Edward E.\} and Hines, \{Oscar J.\} and Reeve, \{Joseph R.\} and Daniel Grandt and Moser, \{James A.\} and Bilchik, \{Anton J.\} and Zinner, \{Michael J.\} and McFadden, \{David W.\} and Ashley, \{Stanley W.\}",

note = "Funding Information: Manuscript received August 23, 1996; revised manuscript received January 30, 1997; accepted February 9, 1997. From the Departments of Surgery and Medicine, UCLA School of Medicine, Los Angeles, California; and Department of Surgery, Brigham and Women{\textquoteright} s Hospital, Boston, Massachusetts. This work was supported by National Institute of Health grants R29-DK-4732 6 (S.W.A.) and R01-DK-3987 0 (M.J.Z.). Address for reprint requests: Dr. Stanley Ashley, Brigham Surgical Group, 110 Cypress St., Brookline, Massachusetts 02146.",

year = "1997",

doi = "10.1023/A:1018869116284",

language = "English",

volume = "42",

pages = "1121--1127",

journal = "Digestive Diseases and Sciences",

issn = "0163-2116",

publisher = "Springer",

number = "6",

}

TY - JOUR

T1 - Antisecretory mechanisms of Peptide YY in rat distal colon

AU - Whang, Edward E.

AU - Hines, Oscar J.

AU - Reeve, Joseph R.

AU - Grandt, Daniel

AU - Moser, James A.

AU - Bilchik, Anton J.

AU - Zinner, Michael J.

AU - McFadden, David W.

AU - Ashley, Stanley W.

N1 - Funding Information: Manuscript received August 23, 1996; revised manuscript received January 30, 1997; accepted February 9, 1997. From the Departments of Surgery and Medicine, UCLA School of Medicine, Los Angeles, California; and Department of Surgery, Brigham and Women’ s Hospital, Boston, Massachusetts. This work was supported by National Institute of Health grants R29-DK-4732 6 (S.W.A.) and R01-DK-3987 0 (M.J.Z.). Address for reprint requests: Dr. Stanley Ashley, Brigham Surgical Group, 110 Cypress St., Brookline, Massachusetts 02146.

PY - 1997

Y1 - 1997

N2 - Peptide YY (PYY) is a potent regulator of intestinal secretion. These studies investigated the role of Y1 and Y2 receptor subtypes in mediating the antisecretory effects of PYY on mucosa-submucosa preparations of rat distal colon. Addition of vasoactive intestinal peptide (VIP) to these tissues resulted in a 140 ± 18% increase in basal short-circuit current (I(sc)) and the induction of Cl secretion. VIP-stimulated increases in I(sc) were abolished by the addition of each of PYY, (Pro34)-PYY, a Y1 receptor- selective agonist, and PYY-(3-36), an endogenous Y2 receptor-selective ligand. However, when tissue neural transmission was blocked with tetrodotoxin, neither PYY nor its receptor subtype-selective analogs were able to inhibit VIP-stimulated increases in I(sc). These results suggest that in the rat distal colon, the antisecretory actions of PYY are mediated through a combination of Y1 and Y2 receptor subtypes or through a novel receptor subtype that is unable to discriminate between (Pro34)-PYY and PYY-(3-36).

AB - Peptide YY (PYY) is a potent regulator of intestinal secretion. These studies investigated the role of Y1 and Y2 receptor subtypes in mediating the antisecretory effects of PYY on mucosa-submucosa preparations of rat distal colon. Addition of vasoactive intestinal peptide (VIP) to these tissues resulted in a 140 ± 18% increase in basal short-circuit current (I(sc)) and the induction of Cl secretion. VIP-stimulated increases in I(sc) were abolished by the addition of each of PYY, (Pro34)-PYY, a Y1 receptor- selective agonist, and PYY-(3-36), an endogenous Y2 receptor-selective ligand. However, when tissue neural transmission was blocked with tetrodotoxin, neither PYY nor its receptor subtype-selective analogs were able to inhibit VIP-stimulated increases in I(sc). These results suggest that in the rat distal colon, the antisecretory actions of PYY are mediated through a combination of Y1 and Y2 receptor subtypes or through a novel receptor subtype that is unable to discriminate between (Pro34)-PYY and PYY-(3-36).

KW - Colon

KW - Intestinal secretion

KW - Neuropeptide Y

KW - Peptide YY

UR - https://www.scopus.com/pages/publications/0030807207

U2 - 10.1023/A:1018869116284

DO - 10.1023/A:1018869116284

M3 - Article

C2 - 9201071

AN - SCOPUS:0030807207

SN - 0163-2116

VL - 42

SP - 1121

EP - 1127

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

IS - 6

ER -

Antisecretory mechanisms of Peptide YY in rat distal colon

Abstract

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