TY - JOUR
T1 - Bifidobacterium infantis 35624 protects against Salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response
AU - Symonds, Erin L.
AU - O'Mahony, Caitlin
AU - Lapthorne, Susan
AU - O'Mahony, David
AU - Sharry, John Mac
AU - O'Mahony, Liam
AU - Shanahan, Fergus
N1 - Funding Information:
Acknowledgements. We would like to acknowledge Jay Radford and Frances O’Brien for their involvement in the care of the mice, and Graham Sherlock for his technical assistance. This work was funded in part by a CSET science foundation Ireland grant. The funding bodies had no involvement in this study.
Funding Information:
Guarantor of the article: Erin L. Symonds, PhD. Specific author contributions: Involved with the design of the trial, collected and analyzed samples throughout all studies, analyzed the data, and drafted and revised the paper: Erin L. Symonds; involved with the design and conduct of the trial, assisted with data analysis, and revised the draft paper: Caitlin O’Mahony; involved with the design and conduct of the trial and revised the draft paper: Susan Lapthorne; involved with the design and conduct of the trial and revised the draft paper: David O’Mahony; involved with the design and conduct of the trial and revised the draft paper: John Mac Sharry; involved with the design of the trial, assisted with data interpretation, and revised the draft paper: Liam O’Mahony; assisted with data interpretation revised the draft paper: Fergus Shanahan. Financial support: This work was supported (salary) by a CJ Martin post-doctoral fellowship (E.L.S.) awarded by the National Health and Medical Research Council of Australia (ID 357702). Potential competing interests: None.
PY - 2012
Y1 - 2012
N2 - OBJECTIVES: Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of Salmonella infection and lipopolysaccharide (LPS) affect brush border enzyme activity in the mouse, and to determine if the probiotic Bifidobacterium longum subspecies infantis 35624 could attenuate the intestinal damage. METHODS: BALB/c mice were challenged with Salmonella enterica serovar Typhimurium UK1 at various doses (10 2-10 8 colony-forming unit (CFU)) and durations (10 6 CFU for 1-6 days). Mice were also treated with B. longum subsp. infantis 35624 for 2 weeks before and during a 6-day S. Typhimurium challenge (10 6 CFU), or before injection of LPS. The small intestine was assessed for morphological changes, mRNA expression of cytokines, and activity of the brush border enzymes sucrase-isomaltase, maltase, and alkaline phosphatase. RESULTS: S. Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05). This also occurred following injection of LPS. Pre-treatment with B. longum subsp. infantis 35624 prevented weight loss, protected brush border enzyme activity, reduced the small intestinal damage, and inhibited the increase in interleukin (IL)-10 and IL-8 expression due to Salmonella challenge. CONCLUSIONS: Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection. B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.
AB - OBJECTIVES: Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of Salmonella infection and lipopolysaccharide (LPS) affect brush border enzyme activity in the mouse, and to determine if the probiotic Bifidobacterium longum subspecies infantis 35624 could attenuate the intestinal damage. METHODS: BALB/c mice were challenged with Salmonella enterica serovar Typhimurium UK1 at various doses (10 2-10 8 colony-forming unit (CFU)) and durations (10 6 CFU for 1-6 days). Mice were also treated with B. longum subsp. infantis 35624 for 2 weeks before and during a 6-day S. Typhimurium challenge (10 6 CFU), or before injection of LPS. The small intestine was assessed for morphological changes, mRNA expression of cytokines, and activity of the brush border enzymes sucrase-isomaltase, maltase, and alkaline phosphatase. RESULTS: S. Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05). This also occurred following injection of LPS. Pre-treatment with B. longum subsp. infantis 35624 prevented weight loss, protected brush border enzyme activity, reduced the small intestinal damage, and inhibited the increase in interleukin (IL)-10 and IL-8 expression due to Salmonella challenge. CONCLUSIONS: Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection. B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.
UR - https://www.scopus.com/pages/publications/84861841822
U2 - 10.1038/ctg.2012.9
DO - 10.1038/ctg.2012.9
M3 - Article
C2 - 23238232
AN - SCOPUS:84861841822
SN - 2155-384X
VL - 3
JO - Clinical and Translational Gastroenterology
JF - Clinical and Translational Gastroenterology
M1 - e15
ER -