Building ProteomeTools based on a complete synthetic human proteome

Daniel P. Zolg; Mathias Wilhelm; Karsten Schnatbaum; Johannes Zerweck; Tobias Knaute; Bernard Delanghe; Derek J. Bailey; Siegfried Gessulat; Hans Christian Ehrlich; Maximilian Weininger; Peng Yu; Judith Schlegl; Karl Kramer; Tobias Schmidt; Ulrike Kusebauch; Eric W. Deutsch; Ruedi Aebersold; Robert L. Moritz; Holger Wenschuh; Thomas Moehring; Stephan Aiche; Andreas Huhmer; Ulf Reimer; Bernhard Kuster

doi:10.1038/nmeth.4153

Building ProteomeTools based on a complete synthetic human proteome

Daniel P. Zolg
, Mathias Wilhelm
, Karsten Schnatbaum
, Johannes Zerweck
, Tobias Knaute
, Bernard Delanghe
, Derek J. Bailey
, Siegfried Gessulat
, Hans Christian Ehrlich
, Maximilian Weininger
, Peng Yu
, Judith Schlegl
, Karl Kramer
, Tobias Schmidt
, Ulrike Kusebauch
, Eric W. Deutsch
, Ruedi Aebersold
, Robert L. Moritz
, Holger Wenschuh
, Thomas Moehring

Stephan Aiche, Andreas Huhmer, Ulf Reimer, Bernhard Kuster

Research output: Contribution to journal › Article › peer-review

201 Scopus citations

Abstract

We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research. Here we report the generation and multimodal liquid chromatography-tandem mass spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical human gene products, and we exemplify the utility of these data in several applications. The resource (available at http://www.proteometools.org) will be extended to >1 million peptides, and all data will be shared with the community via ProteomicsDB and ProteomeXchange.

Original language	English
Pages (from-to)	259-262
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.4153
State	Published - Feb 28 2017

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10.1038/nmeth.4153

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Zolg, D. P., Wilhelm, M., Schnatbaum, K., Zerweck, J., Knaute, T., Delanghe, B., Bailey, D. J., Gessulat, S., Ehrlich, H. C., Weininger, M., Yu, P., Schlegl, J., Kramer, K., Schmidt, T., Kusebauch, U., Deutsch, E. W., Aebersold, R., Moritz, R. L., Wenschuh, H., ... Kuster, B. (2017). Building ProteomeTools based on a complete synthetic human proteome. Nature Methods, 14(3), 259-262. https://doi.org/10.1038/nmeth.4153

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title = "Building ProteomeTools based on a complete synthetic human proteome",

abstract = "We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research. Here we report the generation and multimodal liquid chromatography-tandem mass spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical human gene products, and we exemplify the utility of these data in several applications. The resource (available at http://www.proteometools.org) will be extended to >1 million peptides, and all data will be shared with the community via ProteomicsDB and ProteomeXchange.",

author = "Zolg, \{Daniel P.\} and Mathias Wilhelm and Karsten Schnatbaum and Johannes Zerweck and Tobias Knaute and Bernard Delanghe and Bailey, \{Derek J.\} and Siegfried Gessulat and Ehrlich, \{Hans Christian\} and Maximilian Weininger and Peng Yu and Judith Schlegl and Karl Kramer and Tobias Schmidt and Ulrike Kusebauch and Deutsch, \{Eric W.\} and Ruedi Aebersold and Moritz, \{Robert L.\} and Holger Wenschuh and Thomas Moehring and Stephan Aiche and Andreas Huhmer and Ulf Reimer and Bernhard Kuster",

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doi = "10.1038/nmeth.4153",

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Zolg, DP, Wilhelm, M, Schnatbaum, K, Zerweck, J, Knaute, T, Delanghe, B, Bailey, DJ, Gessulat, S, Ehrlich, HC, Weininger, M, Yu, P, Schlegl, J, Kramer, K, Schmidt, T, Kusebauch, U, Deutsch, EW, Aebersold, R, Moritz, RL, Wenschuh, H, Moehring, T, Aiche, S, Huhmer, A, Reimer, U & Kuster, B 2017, 'Building ProteomeTools based on a complete synthetic human proteome', Nature Methods, vol. 14, no. 3, pp. 259-262. https://doi.org/10.1038/nmeth.4153

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T1 - Building ProteomeTools based on a complete synthetic human proteome

AU - Zolg, Daniel P.

AU - Wilhelm, Mathias

AU - Schnatbaum, Karsten

AU - Zerweck, Johannes

AU - Knaute, Tobias

AU - Delanghe, Bernard

AU - Bailey, Derek J.

AU - Gessulat, Siegfried

AU - Ehrlich, Hans Christian

AU - Weininger, Maximilian

AU - Yu, Peng

AU - Schlegl, Judith

AU - Kramer, Karl

AU - Schmidt, Tobias

AU - Kusebauch, Ulrike

AU - Deutsch, Eric W.

AU - Aebersold, Ruedi

AU - Moritz, Robert L.

AU - Wenschuh, Holger

AU - Moehring, Thomas

AU - Aiche, Stephan

AU - Huhmer, Andreas

AU - Reimer, Ulf

AU - Kuster, Bernhard

PY - 2017/2/28

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AB - We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research. Here we report the generation and multimodal liquid chromatography-tandem mass spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical human gene products, and we exemplify the utility of these data in several applications. The resource (available at http://www.proteometools.org) will be extended to >1 million peptides, and all data will be shared with the community via ProteomicsDB and ProteomeXchange.

UR - https://www.scopus.com/pages/publications/85010951258

U2 - 10.1038/nmeth.4153

DO - 10.1038/nmeth.4153

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SN - 1548-7091

VL - 14

SP - 259

EP - 262

JO - Nature Methods

JF - Nature Methods

IS - 3

ER -

Building ProteomeTools based on a complete synthetic human proteome

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