Growth inhibition and modulation of cell markers of melanoma by s-allyl cysteine

Hiromitsu Takeyama; Dave S.B. Hoon; Romaine E. Saxton; Donald L. Morton; Reiko F. Irie

doi:10.1159/000227149

Growth inhibition and modulation of cell markers of melanoma by s-allyl cysteine

Hiromitsu Takeyama
, Dave S.B. Hoon
, Romaine E. Saxton
, Donald L. Morton
, Reiko F. Irie

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

A sulfur-containing amino acid compound. S-allyl cysteine (SAC), derived from garlic extract inhibited proliferation of nine human and a murine mela-noma cell line in a dose-dependent manner (1.2-10 mM) assessed by a [³H]thy- midine incorporation assay. Three control human lymphoblastoid cell lines were not inhibited by SAC concentrations < 5 mM. Four human melanoma cell lines in a soft-agar assay also showed dose-dependent inhibition of colony formation by SAC. Melanin content was increased up to 95% compared to the same untreated cell lines in these four human melanoma and two B16 murine melanoma sublines. Expression of cell surface gangliosides, cellular-difieren- tiation and transformation markers, decreased after SAC treatment. Significant morphological changes including‘flattening and/or dendritic-like elongations' were also observed. Thus SAC inhibited cellular growth and proliferation and modulated major cell differentiation markers of melanoma

Original language	English
Pages (from-to)	63-69
Number of pages	7
Journal	Oncology (Switzerland)
Volume	50
Issue number	1
DOIs	https://doi.org/10.1159/000227149
State	Published - 1987

Keywords

Gangliosides
Growth inhibition
Melanoma
S-allyl cysteine

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10.1159/000227149

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title = "Growth inhibition and modulation of cell markers of melanoma by s-allyl cysteine",

abstract = "A sulfur-containing amino acid compound. S-allyl cysteine (SAC), derived from garlic extract inhibited proliferation of nine human and a murine mela-noma cell line in a dose-dependent manner (1.2-10 mM) assessed by a [3H]thy- midine incorporation assay. Three control human lymphoblastoid cell lines were not inhibited by SAC concentrations < 5 mM. Four human melanoma cell lines in a soft-agar assay also showed dose-dependent inhibition of colony formation by SAC. Melanin content was increased up to 95\% compared to the same untreated cell lines in these four human melanoma and two B16 murine melanoma sublines. Expression of cell surface gangliosides, cellular-difieren- tiation and transformation markers, decreased after SAC treatment. Significant morphological changes including{\textquoteleft}flattening and/or dendritic-like elongations' were also observed. Thus SAC inhibited cellular growth and proliferation and modulated major cell differentiation markers of melanoma",

keywords = "Gangliosides, Growth inhibition, Melanoma, S-allyl cysteine",

author = "Hiromitsu Takeyama and Hoon, \{Dave S.B.\} and Saxton, \{Romaine E.\} and Morton, \{Donald L.\} and Irie, \{Reiko F.\}",

year = "1987",

doi = "10.1159/000227149",

language = "English",

volume = "50",

pages = "63--69",

journal = "Oncology (Switzerland)",

issn = "0030-2414",

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number = "1",

}

TY - JOUR

T1 - Growth inhibition and modulation of cell markers of melanoma by s-allyl cysteine

AU - Takeyama, Hiromitsu

AU - Hoon, Dave S.B.

AU - Saxton, Romaine E.

AU - Morton, Donald L.

AU - Irie, Reiko F.

PY - 1987

Y1 - 1987

N2 - A sulfur-containing amino acid compound. S-allyl cysteine (SAC), derived from garlic extract inhibited proliferation of nine human and a murine mela-noma cell line in a dose-dependent manner (1.2-10 mM) assessed by a [3H]thy- midine incorporation assay. Three control human lymphoblastoid cell lines were not inhibited by SAC concentrations < 5 mM. Four human melanoma cell lines in a soft-agar assay also showed dose-dependent inhibition of colony formation by SAC. Melanin content was increased up to 95% compared to the same untreated cell lines in these four human melanoma and two B16 murine melanoma sublines. Expression of cell surface gangliosides, cellular-difieren- tiation and transformation markers, decreased after SAC treatment. Significant morphological changes including‘flattening and/or dendritic-like elongations' were also observed. Thus SAC inhibited cellular growth and proliferation and modulated major cell differentiation markers of melanoma

AB - A sulfur-containing amino acid compound. S-allyl cysteine (SAC), derived from garlic extract inhibited proliferation of nine human and a murine mela-noma cell line in a dose-dependent manner (1.2-10 mM) assessed by a [3H]thy- midine incorporation assay. Three control human lymphoblastoid cell lines were not inhibited by SAC concentrations < 5 mM. Four human melanoma cell lines in a soft-agar assay also showed dose-dependent inhibition of colony formation by SAC. Melanin content was increased up to 95% compared to the same untreated cell lines in these four human melanoma and two B16 murine melanoma sublines. Expression of cell surface gangliosides, cellular-difieren- tiation and transformation markers, decreased after SAC treatment. Significant morphological changes including‘flattening and/or dendritic-like elongations' were also observed. Thus SAC inhibited cellular growth and proliferation and modulated major cell differentiation markers of melanoma

KW - Gangliosides

KW - Growth inhibition

KW - Melanoma

KW - S-allyl cysteine

UR - https://www.scopus.com/pages/publications/0027392852

U2 - 10.1159/000227149

DO - 10.1159/000227149

M3 - Article

C2 - 8421600

AN - SCOPUS:0027392852

SN - 0030-2414

VL - 50

SP - 63

EP - 69

JO - Oncology (Switzerland)

JF - Oncology (Switzerland)

IS - 1

ER -

Growth inhibition and modulation of cell markers of melanoma by s-allyl cysteine

Abstract

Keywords

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