Immunological profiling of tumor-infiltrating CD8+ T lymphocytes in primary non-small cell lung cancer (NSCLC), head and neck squamous cell carcinoma (HNSCC), breast cancer (BCa), and renal cell cancers (RCC).

Miller W; William L. Redmond; See full list of authors in comments

Immunological profiling of tumor-infiltrating CD8+ T lymphocytes in primary non-small cell lung cancer (NSCLC), head and neck squamous cell carcinoma (HNSCC), breast cancer (BCa), and renal cell cancers (RCC).

Miller W, William L. Redmond, See full list of authors in comments

Research output: Other contribution

Abstract

To explore whether the variation in clinical response to immune checkpoint blockade (ICB) reflects intrinsic characteristics of tumor-infiltrating lymphocytes (TIL), TILs from multiple tumor types were analyzed by multiparameter flow cytometry. Recent work identified CD39+CD103+ double positive (DP) CD8 TIL as tumor-reactive, therefore we assessed this phenotype in primary NSCLC (n=28), BCa (n=23), HNSCC (n=23), and RCC (n=23) specimens. TILs from NSCLC and HNSCC had significantly higher frequencies of DP T cells than BCa and RCC (NSCLC: median=34.2, IQR=15.0-53.8%; HNSCC: median=28.6%, IQR=13.5-44.5%), while TILs from BCa and RCC had low frequencies of DP T cells (BCa: median=2.4%, IQR=1.1-3.9%; RCC: median=4.2%, IQR=1.5-16.6%). Additionally, DP cells in NSCLC and HNSCC were co-expressed immune checkpoint markers PD-1, TIM-3, and LAG-3 at a higher frequency than RCC or BCa (P

Original language	Undefined/Unknown
State	Published - May 1 2022

Publication series

Name	Articles, Abstracts, and Reports

Access to Document

http://digitalcommons.psjhealth.org/publications/6122

Cite this

@misc{5c1808d0337248d19150a805681f9a41,

title = "Immunological profiling of tumor-infiltrating CD8+ T lymphocytes in primary non-small cell lung cancer (NSCLC), head and neck squamous cell carcinoma (HNSCC), breast cancer (BCa), and renal cell cancers (RCC).",

abstract = "To explore whether the variation in clinical response to immune checkpoint blockade (ICB) reflects intrinsic characteristics of tumor-infiltrating lymphocytes (TIL), TILs from multiple tumor types were analyzed by multiparameter flow cytometry. Recent work identified CD39+CD103+ double positive (DP) CD8 TIL as tumor-reactive, therefore we assessed this phenotype in primary NSCLC (n=28), BCa (n=23), HNSCC (n=23), and RCC (n=23) specimens. TILs from NSCLC and HNSCC had significantly higher frequencies of DP T cells than BCa and RCC (NSCLC: median=34.2, IQR=15.0-53.8%; HNSCC: median=28.6%, IQR=13.5-44.5%), while TILs from BCa and RCC had low frequencies of DP T cells (BCa: median=2.4%, IQR=1.1-3.9%; RCC: median=4.2%, IQR=1.5-16.6%). Additionally, DP cells in NSCLC and HNSCC were co-expressed immune checkpoint markers PD-1, TIM-3, and LAG-3 at a higher frequency than RCC or BCa (P ",

author = "Miller W and Redmond, {William L.} and comments, {See full list of authors in}",

year = "2022",

month = may,

day = "1",

language = "Undefined/Unknown",

series = "Articles, Abstracts, and Reports",

type = "Other",

}

TY - GEN

T1 - Immunological profiling of tumor-infiltrating CD8+ T lymphocytes in primary non-small cell lung cancer (NSCLC), head and neck squamous cell carcinoma (HNSCC), breast cancer (BCa), and renal cell cancers (RCC).

AU - W, Miller

AU - Redmond, William L.

AU - comments, See full list of authors in

PY - 2022/5/1

Y1 - 2022/5/1

N2 - To explore whether the variation in clinical response to immune checkpoint blockade (ICB) reflects intrinsic characteristics of tumor-infiltrating lymphocytes (TIL), TILs from multiple tumor types were analyzed by multiparameter flow cytometry. Recent work identified CD39+CD103+ double positive (DP) CD8 TIL as tumor-reactive, therefore we assessed this phenotype in primary NSCLC (n=28), BCa (n=23), HNSCC (n=23), and RCC (n=23) specimens. TILs from NSCLC and HNSCC had significantly higher frequencies of DP T cells than BCa and RCC (NSCLC: median=34.2, IQR=15.0-53.8%; HNSCC: median=28.6%, IQR=13.5-44.5%), while TILs from BCa and RCC had low frequencies of DP T cells (BCa: median=2.4%, IQR=1.1-3.9%; RCC: median=4.2%, IQR=1.5-16.6%). Additionally, DP cells in NSCLC and HNSCC were co-expressed immune checkpoint markers PD-1, TIM-3, and LAG-3 at a higher frequency than RCC or BCa (P

AB - To explore whether the variation in clinical response to immune checkpoint blockade (ICB) reflects intrinsic characteristics of tumor-infiltrating lymphocytes (TIL), TILs from multiple tumor types were analyzed by multiparameter flow cytometry. Recent work identified CD39+CD103+ double positive (DP) CD8 TIL as tumor-reactive, therefore we assessed this phenotype in primary NSCLC (n=28), BCa (n=23), HNSCC (n=23), and RCC (n=23) specimens. TILs from NSCLC and HNSCC had significantly higher frequencies of DP T cells than BCa and RCC (NSCLC: median=34.2, IQR=15.0-53.8%; HNSCC: median=28.6%, IQR=13.5-44.5%), while TILs from BCa and RCC had low frequencies of DP T cells (BCa: median=2.4%, IQR=1.1-3.9%; RCC: median=4.2%, IQR=1.5-16.6%). Additionally, DP cells in NSCLC and HNSCC were co-expressed immune checkpoint markers PD-1, TIM-3, and LAG-3 at a higher frequency than RCC or BCa (P

M3 - Other contribution

T3 - Articles, Abstracts, and Reports

ER -