NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulates normoxic HIF-1α and p27

Smitha Antony; Guojian Jiang; Yongzhong Wu; Jennifer L Meitzler; Hala R Makhlouf; Diana C Haines; Donna Butcher; Dave S B Hoon; Jiuping Ji; Yiping Zhang; Agnes Juhasz; Jiamo Lu; Han Liu; Iris Dahan; Mariam Konate; Krishnendu K Roy; James H Doroshow

NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulates normoxic HIF-1α and p27

Smitha Antony
, Guojian Jiang
, Yongzhong Wu
, Jennifer L Meitzler
, Hala R Makhlouf
, Diana C Haines
, Donna Butcher
, Dave S B Hoon
, Jiuping Ji
, Yiping Zhang
, Agnes Juhasz
, Jiamo Lu
, Han Liu
, Iris Dahan
, Mariam Konate
, Krishnendu K Roy
, James H Doroshow

California

Research output: Contribution to journal › Article

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Abstract

NADPH oxidase 5 (NOX5) generated reactive oxygen species (ROS) have been implicated in signaling cascades that regulate cancer cell proliferation. To evaluate and validate NOX5 expression in human tumors, we screened a broad range of tissue microarrays (TMAs), and report substantial overexpression of NOX5 in malignant melanoma and cancers of the prostate, breast, and ovary. In human UACC-257 melanoma cells that possesses high levels of functional endogenous NOX5, overexpression of NOX5 resulted in enhanced cell growth, increased numbers of BrdU positive cells, and increased γ-H2AX levels. Additionally, NOX5-overexpressing (stable and inducible) UACC-257 cells demonstrated increased normoxic HIF-1α expression and decreased p27

Original language	Undefined/Unknown
Journal	Articles, Abstracts, and Reports
State	Published - Dec 1 2017

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NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulate

http://digitalcommons.providence.org/publications/2314

Cite this

Antony, S., Jiang, G., Wu, Y., Meitzler, J. L., Makhlouf, H. R., Haines, D. C., Butcher, D., Hoon, D. S. B., Ji, J., Zhang, Y., Juhasz, A., Lu, J., Liu, H., Dahan, I., Konate, M., Roy, K. K., & Doroshow, J. H. (2017). NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulates normoxic HIF-1α and p27. Articles, Abstracts, and Reports. http://digitalcommons.providence.org/publications/2314

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title = "NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulates normoxic HIF-1α and p27",

abstract = "NADPH oxidase 5 (NOX5) generated reactive oxygen species (ROS) have been implicated in signaling cascades that regulate cancer cell proliferation. To evaluate and validate NOX5 expression in human tumors, we screened a broad range of tissue microarrays (TMAs), and report substantial overexpression of NOX5 in malignant melanoma and cancers of the prostate, breast, and ovary. In human UACC-257 melanoma cells that possesses high levels of functional endogenous NOX5, overexpression of NOX5 resulted in enhanced cell growth, increased numbers of BrdU positive cells, and increased γ-H2AX levels. Additionally, NOX5-overexpressing (stable and inducible) UACC-257 cells demonstrated increased normoxic HIF-1α expression and decreased p27",

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journal = "Articles, Abstracts, and Reports",

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TY - JOUR

T1 - NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulates normoxic HIF-1α and p27

AU - Antony, Smitha

AU - Jiang, Guojian

AU - Wu, Yongzhong

AU - Meitzler, Jennifer L

AU - Makhlouf, Hala R

AU - Haines, Diana C

AU - Butcher, Donna

AU - Hoon, Dave S B

AU - Ji, Jiuping

AU - Zhang, Yiping

AU - Juhasz, Agnes

AU - Lu, Jiamo

AU - Liu, Han

AU - Dahan, Iris

AU - Konate, Mariam

AU - Roy, Krishnendu K

AU - Doroshow, James H

PY - 2017/12/1

Y1 - 2017/12/1

N2 - NADPH oxidase 5 (NOX5) generated reactive oxygen species (ROS) have been implicated in signaling cascades that regulate cancer cell proliferation. To evaluate and validate NOX5 expression in human tumors, we screened a broad range of tissue microarrays (TMAs), and report substantial overexpression of NOX5 in malignant melanoma and cancers of the prostate, breast, and ovary. In human UACC-257 melanoma cells that possesses high levels of functional endogenous NOX5, overexpression of NOX5 resulted in enhanced cell growth, increased numbers of BrdU positive cells, and increased γ-H2AX levels. Additionally, NOX5-overexpressing (stable and inducible) UACC-257 cells demonstrated increased normoxic HIF-1α expression and decreased p27

AB - NADPH oxidase 5 (NOX5) generated reactive oxygen species (ROS) have been implicated in signaling cascades that regulate cancer cell proliferation. To evaluate and validate NOX5 expression in human tumors, we screened a broad range of tissue microarrays (TMAs), and report substantial overexpression of NOX5 in malignant melanoma and cancers of the prostate, breast, and ovary. In human UACC-257 melanoma cells that possesses high levels of functional endogenous NOX5, overexpression of NOX5 resulted in enhanced cell growth, increased numbers of BrdU positive cells, and increased γ-H2AX levels. Additionally, NOX5-overexpressing (stable and inducible) UACC-257 cells demonstrated increased normoxic HIF-1α expression and decreased p27

M3 - Article

JO - Articles, Abstracts, and Reports

JF - Articles, Abstracts, and Reports

ER -