Pig kidney (LLC-PK1) cell membrane fluidity during exposure to gentamicin or tobramycin

S. J. Kohlhepp; L. Hou; D. N. Gilbert

doi:10.1128/AAC.38.9.2169

Pig kidney (LLC-PK₁) cell membrane fluidity during exposure to gentamicin or tobramycin

S. J. Kohlhepp
, L. Hou
, D. N. Gilbert

Research output: Contribution to journal › Comment/debate

6 Scopus citations

Abstract

The surface membrane properties of LLC-PK₁ cells grown with and without various amounts of gentamicin or tobramycin for various lengths of time were determined by measuring the diffusion coefficient of N-(7-nitrobenz-2-oxa- 1,3-diazol-4-yl)dipalmitoyl-L-α-phosphatidylethanolamine (NBD-PE) and the percentage of NBD-PE free to diffuse after photobleaching. One hour of exposure to tobramycin decreased the percentage that was free to diffuse. After 1 day or longer of exposure to either aminoglycoside the percentage that was free to diffuse returned to preexposure levels and the diffusion coefficient decreased.

Original language	English
Pages (from-to)	2169-2171
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	38
Issue number	9
DOIs	https://doi.org/10.1128/AAC.38.9.2169
State	Published - 1994

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title = "Pig kidney (LLC-PK1) cell membrane fluidity during exposure to gentamicin or tobramycin",

abstract = "The surface membrane properties of LLC-PK1 cells grown with and without various amounts of gentamicin or tobramycin for various lengths of time were determined by measuring the diffusion coefficient of N-(7-nitrobenz-2-oxa- 1,3-diazol-4-yl)dipalmitoyl-L-α-phosphatidylethanolamine (NBD-PE) and the percentage of NBD-PE free to diffuse after photobleaching. One hour of exposure to tobramycin decreased the percentage that was free to diffuse. After 1 day or longer of exposure to either aminoglycoside the percentage that was free to diffuse returned to preexposure levels and the diffusion coefficient decreased.",

author = "Kohlhepp, \{S. J.\} and L. Hou and Gilbert, \{D. N.\}",

year = "1994",

doi = "10.1128/AAC.38.9.2169",

language = "English",

volume = "38",

pages = "2169--2171",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "9",

}

TY - JOUR

T1 - Pig kidney (LLC-PK1) cell membrane fluidity during exposure to gentamicin or tobramycin

AU - Kohlhepp, S. J.

AU - Hou, L.

AU - Gilbert, D. N.

PY - 1994

Y1 - 1994

N2 - The surface membrane properties of LLC-PK1 cells grown with and without various amounts of gentamicin or tobramycin for various lengths of time were determined by measuring the diffusion coefficient of N-(7-nitrobenz-2-oxa- 1,3-diazol-4-yl)dipalmitoyl-L-α-phosphatidylethanolamine (NBD-PE) and the percentage of NBD-PE free to diffuse after photobleaching. One hour of exposure to tobramycin decreased the percentage that was free to diffuse. After 1 day or longer of exposure to either aminoglycoside the percentage that was free to diffuse returned to preexposure levels and the diffusion coefficient decreased.

AB - The surface membrane properties of LLC-PK1 cells grown with and without various amounts of gentamicin or tobramycin for various lengths of time were determined by measuring the diffusion coefficient of N-(7-nitrobenz-2-oxa- 1,3-diazol-4-yl)dipalmitoyl-L-α-phosphatidylethanolamine (NBD-PE) and the percentage of NBD-PE free to diffuse after photobleaching. One hour of exposure to tobramycin decreased the percentage that was free to diffuse. After 1 day or longer of exposure to either aminoglycoside the percentage that was free to diffuse returned to preexposure levels and the diffusion coefficient decreased.

UR - https://www.scopus.com/pages/publications/0028024040

U2 - 10.1128/AAC.38.9.2169

DO - 10.1128/AAC.38.9.2169

M3 - Comment/debate

C2 - 7811038

AN - SCOPUS:0028024040

SN - 0066-4804

VL - 38

SP - 2169

EP - 2171

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 9

ER -

Pig kidney (LLC-PK₁) cell membrane fluidity during exposure to gentamicin or tobramycin

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