Prediction strategy for screening functional Haloarchaea strains with qPCR assays

Xinyu Hu; Wenxiang Sun; Meng Zhang; Wenjun Guo; Shujing Yang; Lin Zhu; Xiang Xiao; Xiangru Xu; Wei Wei

doi:10.1016/j.mimet.2024.107029

Prediction strategy for screening functional Haloarchaea strains with qPCR assays

Xinyu Hu
, Wenxiang Sun
, Meng Zhang
, Wenjun Guo
, Shujing Yang
, Lin Zhu
, Xiang Xiao
, Xiangru Xu
, Wei Wei

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

As an extremophile resource, functional Haloarchaea strains are extremely time-consuming to screen. Here, taking the screening of low-salt-tolerant strains as an example, based on the qPCR assays that shortened time by 4–7 times and achieved 100 % accuracy, a universal strategy for rapid and accurate screening of functional Haloarchaea strains was established.

Original language	English
Article number	107029
Journal	Journal of Microbiological Methods
Volume	226
DOIs	https://doi.org/10.1016/j.mimet.2024.107029
State	Published - Nov 2024
Externally published	Yes

Keywords

Haloarchaea
Strain screening
qPCR

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10.1016/j.mimet.2024.107029

Cite this

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title = "Prediction strategy for screening functional Haloarchaea strains with qPCR assays",

abstract = "As an extremophile resource, functional Haloarchaea strains are extremely time-consuming to screen. Here, taking the screening of low-salt-tolerant strains as an example, based on the qPCR assays that shortened time by 4–7 times and achieved 100 \% accuracy, a universal strategy for rapid and accurate screening of functional Haloarchaea strains was established.",

keywords = "Haloarchaea, Strain screening, qPCR",

author = "Xinyu Hu and Wenxiang Sun and Meng Zhang and Wenjun Guo and Shujing Yang and Lin Zhu and Xiang Xiao and Xiangru Xu and Wei Wei",

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T1 - Prediction strategy for screening functional Haloarchaea strains with qPCR assays

AU - Hu, Xinyu

AU - Sun, Wenxiang

AU - Zhang, Meng

AU - Guo, Wenjun

AU - Yang, Shujing

AU - Zhu, Lin

AU - Xiao, Xiang

AU - Xu, Xiangru

AU - Wei, Wei

PY - 2024/11

Y1 - 2024/11

N2 - As an extremophile resource, functional Haloarchaea strains are extremely time-consuming to screen. Here, taking the screening of low-salt-tolerant strains as an example, based on the qPCR assays that shortened time by 4–7 times and achieved 100 % accuracy, a universal strategy for rapid and accurate screening of functional Haloarchaea strains was established.

AB - As an extremophile resource, functional Haloarchaea strains are extremely time-consuming to screen. Here, taking the screening of low-salt-tolerant strains as an example, based on the qPCR assays that shortened time by 4–7 times and achieved 100 % accuracy, a universal strategy for rapid and accurate screening of functional Haloarchaea strains was established.

KW - Haloarchaea

KW - Strain screening

KW - qPCR

UR - https://www.scopus.com/pages/publications/85203404307

U2 - 10.1016/j.mimet.2024.107029

DO - 10.1016/j.mimet.2024.107029

M3 - Article

C2 - 39245367

AN - SCOPUS:85203404307

SN - 0167-7012

VL - 226

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

M1 - 107029

ER -

Prediction strategy for screening functional Haloarchaea strains with qPCR assays

Abstract

Keywords

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